Technical Data
B0902-02A1
BD3 (beta Defensin-3, CTIP1) (HRP)
Description:
A novel, nonhemolytic antimicrobial peptide (human beta-defensin-3, hBD-3) was isolated from human lesional psoriatic scales and clones from keratinocytes. hBD-3 demonstrated a salt-insensitive broad spectrum of potent antimicrobial activity against many potentially pathogenic microbes including multiresistant S. aureus and vancomyosin-resistant Entoerococcus faecium. Keratinocytes and airway epithelial cells are cellular sources of hBD-3. Tumor necrosis factor alpha and contact with bacteria were found to induce hBD-3 mRNA expression. hBD-3, therefore, might be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung, such as cystic fibrosis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000 (ECL)
Immunofluorescence: 1:100-1:200 (Suggested; not tested)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store at 4°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to residues 23-33 [GIINTLQKYYC] of hBD-3 protein. MW of Antigen: 5kD
Purity:
Purified IgG
Form
Supplied as a liquid in PBS, pH 7.2. No preservative and no stabilizing protein added. Conjugated to horseradish peroxidase (HRP).
Specificity:
Recognizes residues 23-33 (GIINTLQKYYC) of the 5 kD hBD-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Harder, J., Bartels, J., Christophers, E. and Schroder, JM, J. Biol Chem. Feb 23; 276(8): 5707-5713 (2001). 2. Supp, DM., et al. Expression of Human Beta-Defensins HBD-1, HBD-2, and HBD-3 in Cultured Keratinocytes and Skin Substitutes. National Library of Medicine. 7:643-8, 2004