Technical Data
B0902-02E
BD3 (Beta-defensin 3, HBD-3, BD-3, Defensin, beta 103A, Defensin, beta 103, Beta-defensin 3, DEFB-3, BD-3, HBD3, Defensin-like Protein, DEFB103, DEFB3)
Description:
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely beta-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.

The genes encoding human alpha and beta defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated and based on distinctive, although similar, tri-disulfide linkages in the peptides. beta-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six defensin cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been -defensins are encoded by genes designated DEFA1-6, whereas human beta-defensins are encoded by the DEFB1 and DEFb2 genes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ul/ml
Western Blot: 1-3ug/ml (ECL)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Recombinant corresponding to full length human HDEFA-1/NP-1 (Accession #P81534).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA.
Specificity:
Recognizes human BD-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bals R et al (1999) Infect. Immun. 67, 3542-3547. 2. Harder et al (1997) Nature 387, 861. 3. Ganz T (1999) Science 286, 420. 4. Yang D et al (1999) Science 286, 525.