Technical Data
B0902-04A
BD3, Control Peptide (beta Defensin-3)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Control peptide for B0902-04 and B0902-05.

Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tridisulfides and a largely beta-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range ofpathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.

The genes encoding human alpha and beta-defensins areclustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfidelinkages in the peptides. beta-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six alpha-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two beta-defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified. Human BD-3 (HBD-3, mature peptide 23-67aa) and mouse BD-3 (MBD-3) are synthesized from 67 and 63aa precursors, respectively.

Source:
Synthetic peptide corresponding to 13aa of mouse MBD-3 and 15aa of HBD-3, N-terminal.

Molecular Weight:
~3kD

Applications:
Suitable for use in ELISA and Antibody Blocking. It is not suitable for use in Western. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~3kD
Source: Synthetic peptide
Purity: Purified
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder from PBS, pH7.5.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Bals R et al (1999) Infect. Immun. 67, 3542-3547. 2. Harder et al (1997) Nature 387, 861. 3. Ganz T (1999) Science 286, 420. 4. Yang D et al (1999) Science 286, 525.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.