Technical Data
B0902B
BD3, Mouse/human, Control Peptide (beta-Defensin 3)
50ug
Molecular Biology Storage: -20°CShipping: Blue Ice
Control Peptide for (affinity purified) and (antiserum).
Synthetic peptide, A 13 aa peptide mapping at the N-terminus of mature mouse defensin-3 and a 15-aa peptide mapping at the N-terminus of mature human defensin-3 were synthesized for antibody production.

Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.

The genes encoding human a and b -defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated a – and b-based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a –defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b -defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified.

Source:
Human beta-defensin-3 is produced from 67aa peptide precursor (mature peptide 23-67aa). Mouse beta-defensin-3 is produced from a 63aa peptide precursor (mature peptide 23-63aa).

Applications:
Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot due to low MW. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml to coat plates.
Antibody Blocking: 5-10ug peptide.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human synthetic peptide
Purity: Purified
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity: Species sequence homology: The defensin-3 peptides used for antibody production are specific for mouse and human defensin-3.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Bals R et al (1999) Infect. Immun. 67, 3542-3547; 2. Harder et al (1997) Nature 387, 861; 3. Ganz T (1999) Science 286, 420;
4. Yang D et al (1999) Science 286, 525.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.