Technical Data
B0903-25
BD4 (beta Defensin-4)
Description:
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.

The genes encoding human a and -defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated a-and -based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a -defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two -defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
ELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml.

Optimal dilutions to be determined by the researcher.

Control Peptide:
B0903-20 BD4, Mouse Control Peptide (beta Defensin-4)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Synthesized from 63aa precurser. An 11aa peptide from MBD-4 sequence near the N-terminus of mature defensin 4, coupled to KLH.
Purity:
Serum
Form
Supplied as a liquid in PBS 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes mouse defensin-4. No significant homology exists with human beta-defensins-4 or other defensins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Jia HP et al (1999) gene accession # AF155882; Harder et al (1997) Nature 387, 861; Ganz T (1999) Science 286, 420; Yang D et al (1999) Science 286, 525.