		Technical Data
B0903-25A	BD4 (beta Defensin-4)

Description:
Defensin beta 4 (BD4), a secreted cationic antimicrobial peptide and member of the beta-defensin family, highly expressed in the testis, which plays an important role in innate immunity. Studies have shown that the expression of BD-4 is up-regulated in respiratory epithelial cells, following infection by both gram-positive and gram-negative bacteria, and that BD-4 acts in synergy with lysozyme and BD-3.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5- 2ug/ml
Western Blot: 0.1- 0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Recombinant human defensin beta 4.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder in PBS, pH7.2. Reconstitute with 100ul sterile ddH2O, 0.09% sodium azide.

Specificity:

Recognizes human defensin beta 4 (BD4).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Conejo-Garcia, J.R.et al. (2001) Human beta-defensin 4: a novel inducible peptide with a specific salt-sensitive spectrum of antimicrobial activity FASEB. J. 15:1819-1821.