Technical Data
B0903-30
BD4 (beta Defensin-4) (Glycerol Free)
Description:
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely beta-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human alpha- and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha- and beta- based on distinctive, although similar, tri-disulfide linkages in the peptides. beta-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six alpha-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two beta-defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control peptide (B0903-20) can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
An 11aa peptide sequence near N-terminus of mature defensin-4. (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes mouse defensin-4. Species Sequence Homology: No significant homology exists with human beta-defensins-4 or other defensins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jia HP et al (1999) gene accession # AF155882. 2. Harder et al (1997) Nature 387, 861. 3. Ganz T (1999) Science 286, 420. 4. Yang D et al (1999) Science 286, 525.