Technical Data
B0981-23E
Beclin-1 (Coiled-coil Myosin-like Bcl-2-Interacting Protein)
Description:
Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60kD coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Raised against an internal synthetic peptide to human Beclin 1, containing residues within 1-100. This sequence has 94% identity with the mouse sequence and 88% identity with the rat sequence.
Purity:
Serum
Form
TBS containing 5 mg/ml BSA as a stabilizer.
Specificity:
This antibody is specific to human Beclin 1. Species Crossreactivity: This antibody can be used for detection of Beclin 1 in human samples. Preliminary results suggest that this antibody may crossreact with mouse protein, as well. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Klionsky, D.J., and Emr, S. Autophagy as a regulated pathway of cellular degradation. Science. 290: 1717-1721, 2000.
2. Liang, X.H., et al. Induction of autophagy and inhibition of tumorogenesis by beclin 1. Nature. 402(6762): 672-676, 1999.
3. Aita, V.M., et al. Cloning and genomic organization of Beclin 1, a candidate tumor suppressor gene on chromosome 17q21. Genomics. 59: 59-65, 1999.
4. Liang, X.H. et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. Journal of Virology. 72(11): 8586-8596, 1998.
5.Massey, Ashish C., et al. Consequences of the selective blockage of chaperone-mediated autophagy. PNAS. 103:5805-5810, 2006