Technical Data
B0981-23V
Beclin-1 (Coiled-coil Myosin-like Bcl-2-Interacting Protein)
Description:
The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in alpha-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (paraffin): 1:50 detects Beclin-1 in human breast tissue.
Immunocytochemistry: 1:50-1:100 detects Beclin-1 in HeLa cells.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
A431 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11C232Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human Beclin-1 near the C-terminus.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% Glycerol.
Specificity:
Recognizes human Beclin-1 near the C-terminus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.