Technical Data
B0981-23Z
Beclin-1 (Coiled-coil Myosin-like BCL2-interacting Protein, Protein GT197, BECN1, GT197)
Description:
Beclin is a Bcl-2 binding protein. Its structure includes a Bcl-2 binding coiled-coil region, and a leucine-rich nuclear export signal (NES). Beclin protein colocalizes with intracytoplasmic organelles and nuclei in normal COS7 and MCF7 cells. Beclin interaction with Bcl-2 may be involved with host viral defense, since over expression of beclin inhibits Sindbis virus replication and expression of beclin lacking the Bcl-2 binding domain has no antiviral effects.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to aa25 from mouse Beclin 1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 50% glycerol.
Specificity:
Recognizes mouse Beclin 1 at ~53kD, ~100kD and ~110kD. Species Crossreactivity: human and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.