Technical Data
B0981-25
Beclin-1, NT (Coiled-coil Myosin-like Bcl-2-Interacting Protein)
Description:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be
defective in tumor cells (1,2). Beclin-1, a coiled-coil Bcl-2- interacting protein homologous to the yeast autophagy gene apg6 (3,4), is a mammalian autophagy gene that can
inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors (5). Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and
mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death (6)

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: .5-1ugml
Optimal dilutions to be determined by the researcher.

Positive Control:
293 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.


TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide Beclin-117aa near the amino terminus of human Beclin-1 (Genbank accession No. AAH10276).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes human Beclin-1. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gozuacik D and Kimchi A. Oncogene. 2004; 23:2891- 906. 2. Kisen GO, et al Carcinogenesis 1993; 14:2501- 5. 3. Liang XH, Ket al J. Virol. 1998; 72:8586-96. 4. Kametaka S, et al J. Biol. Chem. 1998; 273:22284-91. 5. Liang XH,et al Nature 1999; 402:672-6. 6. Pattingre S, et al Cell 2005; 122:927-39. (WD1005)