Technical Data
B1074
Benzo[a]pyrenediol Epoxide (BPDE)
Description:
A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzo[a]pyrene is a representitive PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic.

Applications:
Can be used for immunopurification and for ELISA.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16A485Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.
Specificity:
Recognizes free BPDE and DNA adducts.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Santella, R et al; Immunohistochemical analysis of polycyclic aromatic hydrocarbon-DNA adducts in breast tumor tissue. Cancer Lett 2000, 154: 143. 2. Mumford, J et al; A sensitive ELISA for detecting polycyclic aromatic hydrocarbon-DNA adducts in human tissue. Mutu Res 1996, 359: 171. 3. Santella, R et al; Monoclonal antibodies to DNA modified by benzo[a]pyrene diol epoxide. Carcinogenesis 1984, 5: 373.