		Technical Data
B1074-15	Benzo[a]pyrenediol Epoxide Modified DNA (BPDE-DNA)

Description:
A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzo[a]pyrene is a representitive PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic.

Applications:
Suitable for use in ELISA, Immunopurification, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen/ Paraffin): 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	6A486	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice		Mouse

Concentration:

~0.1mg/ml

Immunogen:

Benzo[a]pyrenediol Epoxide Modified DNA (BPDE-DNA)

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.

Specificity:

Recognizes BPDE-I-DNA (PAH-DNA).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Santella, R et al; Immunohistochemical analysis of polycyclic aromatic hydrocarbon-DNA adducts in breast tumor tissue. Cancer Lett 2000, 154: 143. 2. Mumford, J et al; A sensitive ELISA for detecting polycyclic aromatic hydrocarbon-DNA adducts in human tissue. Mutu Res 1996, 359: 171. 3. Santella, R et al; Monoclonal antibodies to DNA modified by benzo[a]pyrene diol epoxide. Carcinogenesis 1984, 5: 373.