Technical Data
B1074A
Benzo[a]pyrene-Diol Epoxide (BPDE)
Description:
Humans are exposed to polycyclic aromatic hydrocarbons (PAHs) from occupational, environmental, and medical sources. Benzo[a]pyrene is a carcinogenic PAH found in cigarette smoke, heavily polluted air and smoked foods. A diol epoxide derivative of benzo[a]pyrene, 7 , 8 -dihydroxyn -9 ,10 -epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE-I) has been implicated as the key mutagenic benzo[a]pyrene metabolite involved in covalent modification of DNA.

Applications:
Suitable for use in ELISA and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k9H69Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
BPDE-I-G coupled to BSA
Purity:
Purified
Form
Supplied as a liquid in PBS, 50% glycerol.
Specificity:
Recognizes both free polycyclic aromatic hydrocarbons (PAHs) and DNA adducts. In an inhibition assay, the binding of B1074A to BPDE adducts was inhibited by BPDE-1-DNA, BPDE-1-dG, BPDE-1 tetraol, but not by BPDE-II-DNA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Santella, R.M. et al. (1984) Carcinogenesis 5:373. 2. Lee, B.M. and R.M. Santella (1988) Carcinogenesis 9:1773. 3. Strickland, P.T., et al.,1994. Carcinogenesis 15:483-487.4. Kang, D.H., et al., 1995. Carcinogenesis 16:1079-1085.5 . Kang , D.H., et al., 1995. Occupational and Environmental Medicine 52:593-599.