Technical Data
Betacellulin, Recombinant, Human (BTC, Probetacellulin)
Molecular Biology Storage: -20CShipping: Blue Ice
Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Human Betacellulin is initially synthesized as a glycosylated 32.0kD transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant human Betacellulin is a 9.0kD monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Biological Activity:
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is 0.05ng/ml.

Specific activity: 2x10e7units/mg.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Country of Origin:

Molecular Weight:
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC analyses. Endoxoxin: 0.1EU/ug
Form: Supplied as lyophilized powder. Reconstitute in sterile ddH2O, to 0.1-1mg/ml, which can then be further diluted to other aqueous solutions

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.