		Technical Data
B1082-12B	Betaine-Homocysteine Methyltransferase (BHMT, Betaine-Homocysteine S-Methyltransferase)

Description:
Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulfur amino acids, choline and betaine.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from BALB/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a,k	7G21	Affinity Purified

Size		Storage	Shipping	Source	Host
50ul		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Recombinant human BHMT (1-406aa) (E. coli)

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

Specificity:

Recognizes human BHMT at ~45kD. Species Crossreactivity: Mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mckeever, M.P., et al., J. Clin. Sci. 81(4): 551 (1991).