Technical Data
Betaine-Homocysteine Methyltransferase, CT (BHMT, Betaine-Homocysteine S-Methyltransferase)
BHMT is a 45kD cytosolic zinc metalloenzyme predominantly expressed in the liver and kidney, which acts as a major regulator of homocysteine homeostasis, by catalyzing the conversion of betaine and homocysteine to dimethylglycine and methionine.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16000
Western Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug4C/-20C (No Glycerol)Blue IceHumanGoat
Synthetic peptide, C-EQQLKELFEKQK, corresponding to from the C-Terminal region of BHMT (NP_001704.1).
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH 7.3, 0.02% sodium azide, 0.5% BSA.
Recognizes an epitope within the C-terminal region of human BHMT (Betaine-homocysteine methyltransferase). Species Crossreactivity: mouse and rat. Species sequence homology: dog.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ou, X. et al. (2007) Inhibition of human betaine-homocysteine methyltransferase expression by S-adenosylmethionine and methylthioadenosine. J. Biochem. 401: 87-96.