Technical Data
B1082-12C
Betaine-Homocysteine Methyltransferase, CT (BHMT, Betaine-Homocysteine S-Methyltransferase)
Description:
BHMT is a 45kD cytosolic zinc metalloenzyme predominantly expressed in the liver and kidney, which acts as a major regulator of homocysteine homeostasis, by catalyzing the conversion of betaine and homocysteine to dimethylglycine and methionine.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16000
Western Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C/-20C (No Glycerol)Blue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, C-EQQLKELFEKQK, corresponding to from the C-Terminal region of BHMT (NP_001704.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.3, 0.02% sodium azide, 0.5% BSA.
Specificity:
Recognizes an epitope within the C-terminal region of human BHMT (Betaine-homocysteine methyltransferase). Species Crossreactivity: mouse and rat. Species sequence homology: dog.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ou, X. et al. (2007) Inhibition of human betaine-homocysteine methyltransferase expression by S-adenosylmethionine and methylthioadenosine. J. Biochem. 401: 87-96.