		Technical Data
B1082-12F	Betaine-homocysteine S-methyltransferase, CT (BHMT)

Description:
Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulfur aa, choline and betaine.

Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:16,000
Western Blot: 1:16,000, 0.1-0.3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-EQQLKELFEKQK from human BHMT at C-terminal.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Specificity:

Recognizes human BHMT. Species sequence homology: Canine, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.