Technical Data
B1089-40A
Biglycan (BGN, Bone/Cartilage Proteoglycan I, DSPG1, PG-S1, SLRR1A)
Description:
The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival. This gene is a candidate gene for the Happle syndrome.

Positive Control: Rat Skin lysate

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000.
Western Blot: 0.1-0.3ug/ml. ~ 38-40kD band observed in Rat Skin lysates (calculated MW of 41.7kD according to rat NP_058783.1 and human NP_001702.1 ).
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, C-DRLAIQFGNYKK, representing the C Terminus of the human protein according to NP_001702.1. Homology: Human, Mouse, Rat, Dog Epitope: DRLAIQFGNYKK
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH7.2, 0.5% BSA.
Specificity:
Species Crossreactivity: Rat. Other species not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Chen WB, Lenschow W, Tiede K, Fischer JW, Kalthoff H, Ungefroren H. Smad4/DPC4-dependent regulation of biglycan gene expression by transforming growth factor-beta in pancreatic tumor cells. J Biol Chem. 2002 Sep 27;277(39):36118-28. Epub 2002 Jul 24. PMID: 12140283