Technical Data
Media
BGJb Medium Modified w/o L-Glutamine, Phenol Red (Powder)
B1091-05A
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components (mg/liter):
Inorganic Salts:
Magnesium Sulfate97.68
Potassium Chloride350
Sodium Acetate50
Sodium Chloride6200
Potassium Phosphate Mono35
Sodium Phosphate Dibasic140
Amino Acids:
L-Arginine•HCl75
L-Cysteine•HCL80.77
L-Histidine•HCl•H2O150
L-Isoleucine30
L-Leucine50
L-Lysine•HCl240
L-Methionine50
L-Phenylalanine50
L-Threonine75
L-Tryptophan40
L-Tyrosine40
L-Valine65
Vitamins:
D-Biotin0.2
Choline Chloride50
Folic Acid0.2
i-Inositol1
Niacin20
p-aminobenzoic acid2
D-Pantothenic Acid•Ca0.5
Pyridoxal-5-Phosphate•H2O0.2146
Riboflavin0.2
Thiamine•HCl2
DL-a-Tocopherol Phosphate•2Na1
Cyanocobalamin0.04
Other:
Calcium Lactate555
D-Glucose5000
Total:13.451g/L
Medium BGJ was originally developed by Biggers, Gwatkin and Judah in the early 1960's at the Wistar Institute. Subsequent studies resulted in a modification designated BGJb which has been used for supporting cultures of cartilaginous embryonic bone. An additional modification, developed by Sylvia Fitton-Jackson at Strangeways Laboratory in England, is further enriched over the prior formulae. Additional amino acids and vitamins, and increased buffering capacity conferred by the phosphates in the Fitton-Jackson modification, create conditions that permit calcification and growth of cartilaginous embryonic bone.


Directions per Liter: Dissolve 13.45g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 3.5g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white to white, homogenous, free flowing powder.

Solubility (13.5g/L): Colorless to light yellow, clear, complete

pH: 6.2 ± 0.4


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Biggers, J.D., Gwatkin, R.B.L., and Hetner, S., Exp. Cell Res. 25:1,41-58 (1961)