Technical Data
Media
BGJb Medium Fitton-Jackson Modification w/L-Glutamine, w/o Phenol Red (Powder)
B1091-05B
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Magnesium Sulfate0.09768
Potassium Chloride0.4
Sodium Acetate0.050
Sodium Chloride6.8
Sodium Phosphate Dibasic0.112
Sodium Phosphate Mono.0.02434
Amino Acids:
L-Alanine0.25
L-Arginine0.175
L-Aspartic Acid0.15
L-Cysteine•HCL•H2O0.1003
L-Glutamine0.2
Glycine0.8
L-Histidine0.15
L-Isoleucine0.03
L-Leucine0.05
L-Lysine•HCl0.24
L-Methionine0.05
L-Phenylalanine0.05
L-Proline0.4
L-Serine0.2
L-Threonine0.075
L-Tryptophan0.04
L-Tyrosine•2Na•2H2O0.05766
DL-Valine0.065
Vitamins:
PABA0.002
Ascorbic Acid, Sodium0.05
D-Biotin0.0002
Choline Bitartrate0.0907
Vitamin B-120.00004
Folic Acid0.0002
myo-Inositol0.0002
Nicotinic Acid0.02
D-Pantothenic Acid•Ca0.0002
Pyridoxal-5-Phosphate0.0002
Riboflavin0.0002
Thiamine•HCl0.004
DL- -Tocopherol Phosphate•2Na0.001
Other:
Calcium Lactate0.555
D-Glucose10
Phenol Red, Sodiumabsent
Total:21.3g/liter
Medium BGJ was originally developed by Biggers, Gwatkin and Judah in the early 1960's at the Wistar Institute. Subsequent studies resulted in a modification designated BGJb which has been used for supporting cultures of cartilaginous embryonic bone. An additional modification, developed by Sylvia Fitton-Jackson at Strangeways Laboratory in England, is further enriched over the prior formulae. Additional amino acids and vitamins, and increased buffering capacity conferred by the phosphates in the Fitton-Jackson modification, create conditions that permit calcification and growth of cartilaginous embryonic bone.


Directions per Liter: Dissolve 21.3 g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 3.5g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White, homogeneous, free flowing powder

Solubility (21.3g/L): Colorless to slight yellow, clear, complete

pH: As reported

Endotoxin: 1EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Biggers, J.D., Gwatkin, R.B.L., Hetner, S., Growth of Embryonic Avian and Mammalian Tibiae on a Relatively Simple Chemically Defined Medium. Exp. Cell Res. 25: 1, 41-58 (1961).