Technical Data
Media
Bile Esculin Agar (Powder)
B1099
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Pancreatic Digest of Gelatin5.0
Esculin1.0
Beef Extract3.0
Oxbile40.0
Ferric Citrate0.5
Agar15.0
Total:64.5g/liter
Differentiation of Group D from non-Group D Streptococci.

Description:
Bile Esculin Agar is prepared according to the formula specified by Swan, and later evaluated by Facklam and Moody. It was concluded that the bile esculin test provided a reliable means of identifying enterococci and differentiation from non-enterococci. Enterococci are bile tolerant and hydrolyze esculin which imparts a dark brown color to the medium. Gram positive bacteria other than enterococci are inhibited by the bile salts.

Storage:
Store sealed bottle containing dehydrated medium at 2 to 30C. Once opened and recapped, place container in low humidity environment at same storage temperature. Protect from moisture and light. Dehydrated medium should be discarded if not free flowing or if color has changed from original light beige color.


Directions: 1. Dissolve 64.5grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121C (15 psi). Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Microbiological Analysis: Escherichia coli (ATCC 25922)

Appearance: Light beige, homogeneous and free flowing

Solubility: Dark amber and turbid to clear.

pH: 6.6 0.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.