Technical Data
Bilirubin Oxidase (Biotin)
Application(s): Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.

Recommended Dilution:
ELISA: 1:1000 to 1:5000
Optimal dilutions to be determined by the researcher.

Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Biotin/Protein Ratio: 1020 BAC molecules per Goat IgG molecule

Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGHighly Purified
100ug-20CBlue IceGoat
~10mg/ml (UV280)
Bilirubin Oxidase [Myrothecium verrucaria]
Puri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as puri ed and partially puri ed Bilirubin Oxidase [Myrothecium verrucaria].
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.