Technical Data
B1750-01A
Biotin (D-Biotin, Vitamin H, Coenzyme R, Biopeiderm)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:5000-1:10,000
Western Blot: 1:1000-1:5000
Immunohistochemistry (Paraffin): 1:200-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k8J332Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Full length Biotin conjugated to KLH.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid.
Specificity:
Recognizes both free biotin and biotin bound to a carrier protein. Does not crossreact with the free carrier protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.