Technical Data
B1750-01M
Biotin (FITC)
Description:
Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:20-1:2000
Immunocytochemistry: 1:50-1:5000
Optimal dilutions to be determined by the researcher.

Absorption Wavelength: 494nm

Emission Wavelength: 518nm

Fluorochrome/Protein Ratio: 8.1 moles FITC per mole of Goat IgG

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceGoat
Concentration:
~1mg/ml
Immunogen:
Biotin
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA (IgG and protease free), 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes free biotin, coupled with KLH, BSA or IgG/Y. No reactivity to KLH or BSA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.