Technical Data
B1750-02
Biotin (Vitamin H, Coenzyme R), Conjugated
Description:
Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer (PBS containing 0.1% merthiolate or a mixture PBS/glycerol).

TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
D-Biotin conjugated to BSA via amide bond
Purity:
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Recognizes protein-linked D-Biotin: 100%. Crossreactivity: Panthotenic acid BSA: 1:50,000-1:100,000; Reduced Thioctic acid-BSA: 1:50,000-1:100,000
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.