Technical Data
B1750-06A
Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)
Description:
This antibody has a very high affinity for biotin and is excellent for use in various amplification techniques. It recognizes both the free and protein- conjugated (either soluble or cell bound) form of biotin. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 2.5-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2D43Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptides with biotin conjugated to BSA.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes biotin in all species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.