Technical Data
B1750-06X
Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (BSA & Azide Free)
Description:
Biotin is used as a growth factor in mammalian cell culture as well as having numerous immunological purification roles in avidin/streptavidin-biotin binding mechanisms. Biotin, also known as vitamin H or B7, is a water-soluble B-complex vitamin which is composed of an ureido(tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a cofactor in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. It plays a role in the citric acid cycle, which is the process by which biochemical energy is generated during aerobic respiration. Biotin not only assists in various metabolic reactions, but also helps to transfer carbon dioxide. Biotin is also helpful in maintaining a steady blood sugar level. Biotin is often recommended for strengthening hair and nails. Consequently, it is found in many cosmetic and health products for the hair and skin. Deficiency is extremely rare, as intestinal bacteria generally produce an excess of the body's daily requirement.

Applications:
Suitable for use in Confocal Microscopy, Flow Cytometry, Image Analysis, In Situ Nucleic Acid Hybridization and Western Blot. Other applications not tested.

Recommended Dilutions:
Note: In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k0.N.30Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Biotin conjugated to BSA.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. No stabilizing proteins and no preservatives added. Also available with BSA and azide. See B1750-06.
Specificity:
Recognizes Biotin, free and protein-conjugated (either soluble or cell bound) forms. Species Crossreactivity: All species
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.