		Technical Data
B1750-10	Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)

Description:
Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, and Immunodiffusion. Also suitable for conjugation and most immunological methods, high titer and speci city.

Recommended Dilution:
ELISA: 1:10,000 to 1:50,000
Immunohistochemistry: 1:1000-1:5000
Western Blot: 1:10,000-1:40,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
500ug		-20°C	Blue Ice		Goat

Concentration:

~1mg/ml (UV280)

Immunogen:

Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

Purity:

Purified by immunoaf nity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc.

Form

Supplied as a sterile-filtered liquid in PBS, pH 7.2, 0.01% sodium azide.

Specificity:

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.