Technical Data
B1750-10
Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)
Description:
Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, and Immunodiffusion. Also suitable for conjugation and most immunological methods, high titer and speci city.

Recommended Dilution:
ELISA: 1:10,000 to 1:50,000
Immunohistochemistry: 1:1000-1:5000
Western Blot: 1:10,000-1:40,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceGoat
Concentration:
~1mg/ml (UV280)
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Purity:
Purified by immunoaf nity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc.
Form
Supplied as a sterile-filtered liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.