Technical Data
B1750-12
Biotin (Vitamin H, Coenzyme R, Bioepiderm)
Description:
Applications:
Suitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:100,000-1:500,000
Western Blot: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
2ml4C (-20C Glycerol)Blue IceGoat
Concentration:
Not determined
Immunogen:
Conjugated to Keyhole Limpet Hemocyanin (KLH)
Purity:
Purified by delipidation and de brination.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 0.01% sodium azide. Reconstitute with 2ml sterile 40-50% glycerol, ddH2O.
Specificity:
Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against biotinylated IgG and Biotinylated Bovine Serum Albumin (BSA).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.