Technical Data
B1750-15
Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (AP)
Description:
Applications:
Suitable for use in ELISA, Western and Dot Blot, Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:1000 to 1:5000
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store at 4 C before opening. DO NOT FREEZE. This product is stable at 4 C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug4C Do Not FreezeBlue IceGoat
Concentration:
~0.9mg/ml
Immunogen:
Biotin (KLH).
Purity:
Purified by immunoaf nity chromatography.
Form
Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol, 10mg/ml BSA, and 0.01% sodium azide, pH 8.0. Labeled with alkaline phosphatase.
Specificity:
RecognizesBiotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum and Biotinylated IgG.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.