Technical Data
B1750-16
Biotin (AP)
Description:
Applications:
Suitable for Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:500 to 1:2500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store at 4 C before opening. DO NOT FREEZE. This product is stable at 4 C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
500ug4C Do Not FreezeBlue IceGoat
Concentration:
~0.5mg/ml (UV280)
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Purity:
Purified by immunoaf nity chromatography.
Form
Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M Magnesium Chloride, 0.001M zinc chloride, pH 8.0, 10mg/ml BSA, 0.01% sodium azide and 50% glycerol. Labeled with alkaline phosphatase.
Specificity:
Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-AP, anti-goat serum and biotinylated IgG. No reaction was observed against anti-pepsin or anti-goat IgG F(c).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.