Technical Data
B1750-17
Biotin (Cy2)
Description:
Applications:
Suitable for use in Immunoflourescence and ELISA (Fluorescent). Other applications not tested.

Recommended Dilution:
Immunoflourescence: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Absorption Wavelength:
489nm

Emission Wavelength:
505nm

Fluorochrome/Protein Ratio:
2.1 moles Cy2 per mole of goat IgG F(ab)2

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceGoat
Concentration:
~1mg/ml
Immunogen:
Biotin (KLH)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Cy2 (Cyanine 2-OSu - Molecular Weight: 897D). Reconstitution: Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity:
Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, biotin conjugated goat IgG and biotin conjugated BSA. No reaction was observed against anti-goat IgG F(c) or anti-Pepsin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.