Technical Data
B1750-17A
Biotin (Cy3.5)
Description:
Applications:
Suitable for use in FLISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption Wavelength: 581nm
Emission Wavelength: 596nm
Fluorochrome/Protein Ratio: 2.2 moles Cy3.5 per mole of Goat IgG F(ab)2

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceGoat
Concentration:
~1mg/ml
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH). Label: Cy3.5 (Cyanine 3.5-OSu) (MW: 1286 daltons)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% Sodium Azide.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotin conjugated Goat IgG and biotin conjugated BSA. No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.