Technical Data
B1750-17B
Biotin (Cy3)
Description:
Applications:
Suitable for use in FLISA and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption Wavelength: 550nm

Emission Wavelength: 570nm

Fluorochrome/Protein Ratio: 5.4 moles Cy3 per mole of Goat IgG F(ab’)2

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
PabF(ab’)2Affinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceGoat
Concentration:
~1mg/ml
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide. Labeled with Cy3.
Specificity:
Recognizes biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, biotin conjugated goat IgG and biotin conjugated BSA. No reaction was observed against anti-goat IgG F(c) or anti-Pepsin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.