Technical Data
B1750-17E
Biotin (Cy5)
Description:
Applications:
Suitable for use in FLISA and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Excitation Wavelength: 650nm

Emission Wavelength: 667nm

Fluorochrome/Protein Ratio: 5.3 moles Cy5 per mole of goat IgG F(ab)2. Molecular weight of label: 975D.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceGoat
Concentration:
~1mg/ml
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Cy5. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes a single precipitin arc against anti-goat serum, biotin conjugated goat IgG and biotin conjugated BSA. No reaction was observed against anti-goat IgG F(c) or anti-pepsin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.