Technical Data
B1750-19
Biotin (PE)
Description:
Applications:
Suitable for ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:50- 1:200
Western Blot: 1:50-1:200
Optimal dilution determined by the researcher.

Molecular Weight:
240kD

Emission Wavelength:
575nm

Excitation Wavelength:
488nm

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml4°C Do not freezeBlue IceGoat
Concentration:
~0.5mg/ml
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-phycoerythrin, Biotin conjugated IgG and Biotin conjugated bovine serum albumin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.