Technical Data
B1750-19
Biotin (PE)
Description:
Applications:
Suitable for ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:50- 1:200
Western Blot: 1:50-1:200
Optimal dilution determined by the researcher.

Molecular Weight:
240kD

Emission Wavelength:
575nm

Excitation Wavelength:
488nm

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml4C Do not freezeBlue IceGoat
Concentration:
~0.5mg/ml
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-phycoerythrin, Biotin conjugated IgG and Biotin conjugated bovine serum albumin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.