Technical Data
Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (FITC)
Biotin is used as a growth factor in mammalian cell culture as well as having numerous immunological purification roles in avidin/streptavidin-biotin binding mechanisms.

Suitable for use in Immunoelectrophoresis, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, F.I.T., FLISA and Flow Cytometry/FACS. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:20-1:2000
Immunocytochemistry: 1:50-1:5000
F.I.T.: Mean fluorescence intensity of a 1:1000 dilution at Ex-488nm (+/-9)/Em-518nm (+/-9) = 6643 MFI’s
FLISA: Mean fluorescent value of 8ug/ml of FITC conjugate when tested against biotinylated Chicken IgG/Y coated at 4ug/ml = 1558 MFI’s
Optimal dilutions to be determined by the researcher.

Fluorescein-5-isothiocyanate (FITC) Amax = 494nm; Emax = 518nm; F/P Ratio = 8.1:1

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. 
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
PabIgGAffinity Purified
1mg-20°CBlue IceGoat
Purified Biotin
Affinity purified using solid phase biotin. ~95% based on SDS-PAGE.
Supplied as a in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (protease/IgG free), 0.05% sodium azide. Labeled to fluorescein isothiocyanate (FITC)
Recognizes free Biotin and Biotin coupled to BSA, KLH or IgG/Y. Two predominant bands are seen at 50 and 24kD in SDS-PAGE. Does not react with free BSA or free KLH.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. The TH & Feltkamp TE Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 18: 865-873 (1970).