Technical Data
B1750-23
Biotin (FITC or DTAF)
Description:
Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption Wavelength: 495nm

Emission Wavelength: 528nm

Fluorochrome/Protein Ratio:
2.7 moles DTAF per mole of Rabbit IgG

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1mg4°C (-20°C Glycerol)Blue IceRabbit
Concentration:
~10mg/ml (UV280)
Immunogen:
Biotin conjugated Keyhole Limpet Hemocyanin (KLH)
Purity:
Puri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
Form
Supplied as a liquid in SBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% thimerosal. Labeled with Diaminotriazynlaminofluorescein (DTAF). (MW: 530 Daltons)
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Albumin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.