Technical Data
B1750-30
Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (HRP)
Description:
Applications:
Suitable for use in Immunoblot (Western or Dot Blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000 to 1:25,000
Western Blot: 1:500- 1:3000.
Immunohistochemistry: 1:300- 1:1500.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug4C (-20C Glycerol)Blue IceGoat
Concentration:
~1mg/ml (UV280)
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Purity:
Purified by immunoaf nity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate, 40% glycerol. Labeled with HRP.
Specificity:
Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, biotinylated bovine serum albumin and Biotinylated IgG.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.