		Technical Data
B1750-31	Biotin (HRP)

Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:10,000
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	F(ab’)2		Affinity Purified

Size		Storage	Shipping	Source	Host
500ug		4°C (-20°C Glycerol)	Blue Ice		Goat

Concentration:

~1mg/ml (UV280)

Immunogen:

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Purity:

Purified by immunoaf nity chromatography, solid phase adsorption, pepsin digestion and chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol.

Specificity:

Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-goat serum, biotinylated IgG. No crossreactivity was observed against anti-pepsin or anti-goat IgG F(c).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.