Technical Data
B1750-31
Biotin (HRP)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:10,000
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
500ug4C (-20C Glycerol)Blue IceGoat
Concentration:
~1mg/ml (UV280)
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Purity:
Purified by immunoaf nity chromatography, solid phase adsorption, pepsin digestion and chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol.
Specificity:
Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-goat serum, biotinylated IgG. No crossreactivity was observed against anti-pepsin or anti-goat IgG F(c).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.