		Technical Data
B1750-35A	Biotin (Texas Red)

Description:
Applications:
Suitable for use in Immunofluorescence Microscopy. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:500-1:2000
Optimal dilution determined by the researcher.

Absorption Wavelength: 596 nm
Emission Wavelength: 620 nm

Fluorochrome/Protein Ratio: 3.1 moles Texas Red per mole of goat IgG

Storage and Stability:
Lyophilized powder may be stored at 4°C. Reconstitute with sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice		Goat

Concentration:

~1.1mg/ml (after reconstitution)

Immunogen:

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a powder lyophilized from a solution containing PBS, pH 7.2, 10mg/ml BSA (Ig and protease-free), and 0.01% sodium azide. Labeled with Texas Red. Reconstitute with 1ml ddH2O.

Specificity:

Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotinylated bovine serum albumin and biotinylated IgG.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.