Technical Data
B1750-35B
Biotin (Texas Red)
Description:
Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption Wavelength: 596nm
Emission Wavelength: 620nm
Fluorochrome/Protein Ratio: 4.0 moles Texas Red per mole of Goat IgG F(ab’)2

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabF(ab’)2Affinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceGoat
Concentration:
~1mg/ml
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin. Label: Texas Red Sulfonyl Chloride (MW 625 daltons)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10 mg/ml BSA, (IgG and Protease free), 0.01% Sodium Azide.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Biotin conjugated IgG. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
J.A. Titus, etal. J. Immunol. Methods 50; 193, 1982