Technical Data
B1750-37
Biotin (TRITC)
Description:
Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)

Absorption Wavelength: 550 nm Emission Wavelength: 570 nm

Fluorochrome/Protein Ratio: 5.0 moles TRITC per mole of Goat IgG

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free
Preservative: 0.01% (w/v) Sodium Azide

TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceGoat
Concentration:
~1mg/ml.
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 15mg/ml BSA, (IgG and Protease free),0.01% sodium azide. Labeled with Tetramethylrhodamine isothiocyanante (TRITC)
Specificity:
Assay by IEP resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.