Suitable for use in ELISA, Western Blot. Other applications not tested.
Optimal dilutions to be determined by the researcher.
3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
RPMI 1640 with 10% fetal calf serum
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Biotinylated denatured ovalbumin.|
|Purified by Protein A/G affinity chromatography from culture supernatant.|
|Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.|
|Recognizes and reacts strongly with biotin. Strong reaction is seen in ELISA with biotin/OA directly coated onto the microtiter well, also when tested in sandwich ELISA in combination with streptavidin. In Western blot, after SDS-PAGE, reacts with biotinylated proteins in both reduced and non-reduced forms.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.|