		Technical Data
B1750-38	Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)

Description:

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:20,000
Optimal dilutions to be determined by the researcher.

Hybridoma:
3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Culture Medium:
RPMI 1640 with 10% fetal calf serum

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a,k	6F40	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice		Mouse

Concentration:

~0.5mg/ml

Immunogen:

Biotinylated denatured ovalbumin.

Purity:

Purified by Protein A/G affinity chromatography from culture supernatant.

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.

Specificity:

Recognizes and reacts strongly with biotin. Strong reaction is seen in ELISA with biotin/OA directly coated onto the microtiter well, also when tested in sandwich ELISA in combination with streptavidin. In Western blot, after SDS-PAGE, reacts with biotinylated proteins in both reduced and non-reduced forms.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.