Technical Data
B1750-38
Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)
Description:

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:20,000
Optimal dilutions to be determined by the researcher.

Hybridoma:
3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Culture Medium:
RPMI 1640 with 10% fetal calf serum

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k6F40Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Biotinylated denatured ovalbumin.
Purity:
Purified by Protein A/G affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes and reacts strongly with biotin. Strong reaction is seen in ELISA with biotin/OA directly coated onto the microtiter well, also when tested in sandwich ELISA in combination with streptavidin. In Western blot, after SDS-PAGE, reacts with biotinylated proteins in both reduced and non-reduced forms.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.