Technical Data
BLK (B lymphoid tyrosine kinase)
BLK ( B lymphoid tyrosine kinase), with 505-amino acid protein (about 56KDa), belongs to the Src non-receptor tyrosine kinases familDifferent subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking.Blk is expressed exclusively by B lymphocytes and it is thought to function in a signal transductory pathway specific to this lineage. B lymphoid expression of an active Blk mutant caused proliferation of B progenitor cells and enhanced responsiveness of these cells to interleukin 7. Thus, sustained activation of Blk induces responses normally associated with the pre-BCR.

Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1: 10,000
Western Blot: 1: 500- 1: 2,000
Immunohistochemistry-Paraffin: 1: 500- 1: 2,000
Immunohistochemistry-Frozen: 1: 500- 1: 2,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceHumanMouse
Ni-NTA purified truncated recombinant BLK expressed in E. coli strain BL21 (DE3)
Recognizes human BLK (B lymphoid tyrosine kinase).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Theresa Tretter, Ashley E. Ross, Dominic I. Dordai J. Exp. Med., Dec 2003; 198: 1863.