Technical Data
B1900-02
BLK (B lymphoid tyrosine kinase)
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Technical Data |
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B1900-02 |
BLK (B lymphoid tyrosine kinase) |
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Description: BLK ( B lymphoid tyrosine kinase), with 505-amino acid protein (about 56KDa), belongs to the Src non-receptor tyrosine kinases familDifferent subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking.Blk is expressed exclusively by B lymphocytes and it is thought to function in a signal transductory pathway specific to this lineage. B lymphoid expression of an active Blk mutant caused proliferation of B progenitor cells and enhanced responsiveness of these cells to interleukin 7. Thus, sustained activation of Blk induces responses normally associated with the pre-BCR. Applications: Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1: 10,000 Western Blot: 1: 500- 1: 2,000 Immunohistochemistry-Paraffin: 1: 500- 1: 2,000 Immunohistochemistry-Frozen: 1: 500- 1: 2,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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