Technical Data
B2049-90J
BIRC2, CT (Baculoviral IAP Repeat-containing Protein 2, C-IAP1, IAP Homolog B, Inhibitor of Apoptosis Protein 2, IAP-2, hIAP-2, hIAP2, RING Finger Protein 48, TNFR2-TRAF-signaling Complex Protein 2, API1, IAP2, MIHB, RNF48)
Description:
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-a by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lung cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to 14aa at the C-terminus of human cIAP.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human cIAP. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schimmer, AD. Et al. Cancer Res. 64, 7183 (2004). 2. Rothe, M. et al. Cell 83, 1243 (1995). 3. Deveraux, QL. et al. EMBO J. 17, 2215 (1998). 4. Li, X. et al. Nature 416, 345 (2002). 5. Yang, Q-H. et al. Genes Dev. 17, 1487 (2003). 6. Yang, QH. et al. J. Biol. Chem. 279, 16963 (2004).