Technical Data
Bismuth Sulfite Agar (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Casein Digest Peptone5.0
Sodium Chloride5.0
Peptic Digest of Animal5.0
Beef Extract5.0
Disodium Phosphate5.0
Ferrous Sulfate0.3
Bismuth Sulfite Indicator8.0
Brilliant Green0.025
Selective medium for isolation of Salmonella.

In 1926 Wilson and Blair used Bismuth and Sodium Sulfite in a culture formulation for the isolation of typhoid-paratyphoid groups of bacteria bacteria. Further modifications of the formula were described by Hajna and Damon. The current recommended formulation is found in the USP. Bismuth and brilliant green dye acts as inhibitors to most enteric bacteria and Gram positive bacteria. Salmonella typhi produces black colonies surrounded by a metallic sheen. Other Salmonella produce green or black colonies. Some Shigella sp. may grow on this medium.

Store the sealed bottle containing the dehydrated medium at 2 to 30.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.

Directions: 1. Dissolve 53grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121C (15 psi). Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Microbiological Analysis: Escherichia coli (ATCC 25922)

Appearance: Light beige, homogeneous and free flowing

Solubility: Greenish-yellow and opaque.

pH: 7.5 0.2

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.