Technical Data
B2103
BKCa Channel (High-conductance Ca2+ activated Potassium Channel, MaxiK Slo, KCNMA1)
Description:
Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:200-1:500 (rat brain membranes)
Preadsorption Control: 3ug fusion protein (B2103A) per 1ug antibody (B2103).
Immunohistochemistry: Rat uterus and brain sections.
Optimal dilutions to be determined by the researcher.

Control Antigen: B2103A (Included)
Epitope Location: Intracellular, C-terminus.
Epitope Sequence: HSSHS SQSSS KKSSS VHSIP STANR PNRPK SRESR DKQNA TRMTR MGQAE KKWFT DEPDN AYPRN IQIKP MSTHM ANQIN QYKST SSLIP PIREV EDEC IKKIF TDV

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~0.4mg/ml
Immunogen:
GST fusion protein corresponding to residues 1097-1196 of mouse Slo (mSlo) (Accession A48206).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.025% sodium azide, 40% glycerol.
Specificity:
Recognizes BKCa Channel. Splice forms sequence homology: Corresponds to the C-terminus of the longer form of BKCa channel alpha subunit. Overlaps by > 40 residues with a shorter form. Species crossreactivity: Rat and mouse. Species sequence homology: Chicken: 91/99; rabbit: 88/99; turtle: 87/99.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Allard, B. et al. (2000) J. Biol. Chem. 275, 25556. 2. Benkunsky, N.A. et al. (2000) J. Biol. Chem. 275, 27712. 3. Rhodes, M.T., et al. (2001) Am. J. Physiol. 280, L1250. 4. Ohi, Y., et al. (2001) J. Physiol. 534, 313. 5. Reeve, H.L., et al. (2001) J. Appl. Physiol. 90, 2249. 6. Xu, W., et al. (2002) Science 298, 1029. 7. Adamson, C.L., et al. (2002) J. Neurosci. 22, 1385. 8. Thebaud, B., et al. (2002) Pediatr. Res. 52, 19.