Technical Data
B2103-06
Slo1 (BKCa, BKCA alpha, BK channel, BK channel alpha subunit, Kcnma1, Potassium Large Conductance Calcium-Activated Channel, subfamily M, alpha member 1)
Description:
Slo1 (Calcium-activated potassium channel subunit alpha-1) may not only be activated by membrane depolarization but may also be by an increase in cytosolic Ca2+. The concentration of cytosolic Mg2+ also activates this potassium channel. Found to play a part in the repolarization of the membrane potential due to the fact that it diminishes the excitatory voltage in the cell.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1ug/ml detects Slo1 on 10ug of rat brain membrane tissue lysate.
Immunohistochemistry: 1:500 detects Slo1 in rat cerebellum tissue.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain membrane tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k11C233Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to the S9-S10 segments of mouse Slo1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150 mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes S9-S10 segments of Slo1. Species Crossreactivity: rat Species Sequence Homology: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Misonou, H., et al. (2006). J Comp Neurol. 496(3):289-302. 2. Butler, A., et al. (1993). Science. 261:221-224. 3. Buniel, M., et al. (2008). J Comp Neuro. 510(4):367-77.