		Technical Data
B2103-06	Slo1 (BKCa, BKCA alpha, BK channel, BK channel alpha subunit, Kcnma1, Potassium Large Conductance Calcium-Activated Channel, subfamily M, alpha member 1)

Description:
Slo1 (Calcium-activated potassium channel subunit alpha-1) may not only be activated by membrane depolarization but may also be by an increase in cytosolic Ca2+. The concentration of cytosolic Mg2+ also activates this potassium channel. Found to play a part in the repolarization of the membrane potential due to the fact that it diminishes the excitatory voltage in the cell.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1ug/ml detects Slo1 on 10ug of rat brain membrane tissue lysate.
Immunohistochemistry: 1:500 detects Slo1 in rat cerebellum tissue.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain membrane tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a,k	11C233	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant protein corresponding to the S9-S10 segments of mouse Slo1.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150 mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes S9-S10 segments of Slo1. Species Crossreactivity: rat Species Sequence Homology: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Misonou, H., et al. (2006). J Comp Neurol. 496(3):289-302. 2. Butler, A., et al. (1993). Science. 261:221-224. 3. Buniel, M., et al. (2008). J Comp Neuro. 510(4):367-77.